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Small Angle X-ray Scattering Initiative for Europe :: Forum
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PostPosted: 2011.02.23 21:29 
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I was wondering if there was an easy way to calculate the molecular weight of your protein from SAXS data. Can someon tell me how to do this? Is there a program that you can run or a formula that you can just plug some numbers into?

Thanks.


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PostPosted: 2011.02.24 10:56 
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hi

If you recorded scattering of a standard (like lisozyme or BSA), you can
calculate MW of your protein using I0.

You can also try SAXSMoW:
http://www.if.sc.usp.br/~saxs/

Or you can estimate MW from excluded volume.

Cheers
Alex


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PostPosted: 2011.02.24 18:54 
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Thanks Alex,

SAXSMoW worked very well for me. I have collected a BSA standard as well and in some cases I have both the lysozyme and BSA standards. Can you please explain how I would calculate the MW using these standards?

Thanks again.


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PostPosted: 2011.02.24 18:57 
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MW(X)/I0(X) = MW(Standard)/I0(Standard)

HTH
Alex


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