SAXS

I've downloaded ATSAS 2.8.4, installed it for a Mac (OS 10.13.6) but can't seem to find primus in the package. My ATSAS is currently stored in the 'Applications' folder. Am I missing something? Yes, you are: Due to incompatibilities of Qt4 with recent versions of MacOSX, there are no GUI programs (...

vas2201 wrote: ↑

2018.09.21 22:05

******* GAJOE - version 2.1 - (r7883) *******

This is a bit outdated. Please download the latest ATSAS version and follow these instructions.

Are you using EOM online or on your PC? Is there an error message?

The fit is okay at low q... It's not okay - the Guinier region is not fitted: the R g of your experimental data is clearly greater than R g of your fit. Does the molecular weight estimated from your experimental data match the molecular weight calculated from your protein sequence? ... but at highe...

Your file appears to be corrupt - the actual data are missing. It comes from an older version of GNOM; make sure you have the latest version of ATSAS installed and reprocess your data following these instructions (steps 1-6).

The ATSAS download page is back online.

Moving to a new FTP server is taking longer than expected. This will be resolved within a few hours. Apologies for the inconvenience.

QUESTION 1: the wild-type and mutant A are fine, different concentrations do not change Rg or Dmax... Mutant_A/S?.dat: from the rather narrow concentration range of 0.6-2.1 mg/ml I'd say there are concentration effects: the Guinier R g increases with concentration from 2.6 to 3.0 nm. The extrapolat...

I ran EOM on my computer with default parameters and it's turned out that they are totally different from those obtained with EOM online. Could you please be more specific? Are you sure you are running the latest version of ATSAS ? EOM online runs with arguments like these: $ eom sequence.seq --qua...

I am working on a two-domain protein. Two domains are connected with a small linker. Structure of one of the domains is solved from one organism and that of other is known from the other organism. You may try CORAL to fit your experimental data with a single model or EOM to fit your data with an en...

I guess you mean the "old" primusqw on Windows. Please download the latest ATSAS version and open your dat files in the "new" primus qt (on Windows it is called "SAS Data Analysis" in the Start menu). The top file will be used as a reference for the 'Merge' and 'Scale' operations (found under the 'P...

Left plot log(I) vs. s : fit of the Fourier transform of p(r) (blue line) to the experimental SAS data (black dots). The fit is computed like this: I(s) = 4Π ∫ o Dmax p(r) sin(sr)/sr dr Right plot p(r) vs. r : pair distance distribution function. Total quality estimate ranges between 1.0 (best) and...

Redo the radial averaging. E.g. if you use pyFAI - switch "pixel splitting" to "No splitting".

I 0 : Forward scattering I(0). R g : Radius of gyration - the average of square center-of-mass distances in the molecule (weighted by the scattering length density). It is a measure for the overall size of a macromolecule. If the experimental data are in inverse angstroms (Å -1 ), R g is provided i...

1. Make sure you have the latest ATSAS version installed. 2. Open your background-subtracted data in PRIMUS (on Windows make sure you use primusqt; it is called "SAS Data Analysis" in the Start menu). 3. In the bottom right panel go to the 'Analysis' tab and click Distance Distribution'. This will s...