Search found 8 matches

by EduardoBruch
2020.08.11 18:55
Forum: Primary data processing
Topic: Parameter to inform quality of the fitting for a Pair distance distribution to raw data
Replies: 2
Views: 467

Re: Parameter to inform quality of the fitting for a Pair distance distribution to raw data

Thanks Al, Indeed I managed to get the CorMap test p-value and the reduced Chi-sq value (attached image). Which one will be best to inform? Also I noticed that the Chi-sq is lower than one so I a ot sure I a not over-interpreting. Thanks again for your help! Bests Eduardo Screen Shot 2020-08-11 at 1...
by EduardoBruch
2020.08.10 14:14
Forum: Primary data processing
Topic: Parameter to inform quality of the fitting for a Pair distance distribution to raw data
Replies: 2
Views: 467

Parameter to inform quality of the fitting for a Pair distance distribution to raw data

Hello all, I am preparign a figure with the the Pair distance distribution function in which I want to inlcude the pair distance distribution per se and also the fitting (middle and left pannel in the attached figure respectively). The fitting looks very good but I was wondering if there is a parame...
by EduardoBruch
2020.04.25 19:27
Forum: Primary data processing
Topic: Molecular weight estimation
Replies: 5
Views: 6122

Re: Molecular weight estimation

Hello all, I have a new question about MW estimation. I am calculating the MW using the tool from primus and I found a bigger than usual difference in the estimation by the different techniques. real MW= 750 Kda, calculate using primus QP=700 Kda, MoW 420 Kda, Vc 442 Kda, size and shape 660 kDa and ...
by EduardoBruch
2020.01.28 12:45
Forum: Ab initio shape determination
Topic: DAMMIN using Symmetry D2
Replies: 3
Views: 1089

Re: DAMMIN using Symmetry D2

Hello Al,
Thanks for your answer. I will try this asap to see how it works. But if I understand correctly by using this command line I will be only imposing P2 symmetry right?
Again thanks for your help.
regards

Eduardo
by EduardoBruch
2020.01.22 22:01
Forum: Ab initio shape determination
Topic: DAMMIN using Symmetry D2
Replies: 3
Views: 1089

DAMMIN using Symmetry D2

Hello
I am doing DAMMIN in some of my proteins and is working very well but I am having problem understanding the symmetry. Is is possible to do symmetry D2?
Thanks in advance

Eduardo
by EduardoBruch
2019.04.30 13:03
Forum: Primary data processing
Topic: Molecular weight estimation
Replies: 5
Views: 6122

Re: Molecular weight estimation

Hello I wanted to share a curiosity I found with some of my data. I am processing the saxs data from 2 homologous proteins from 2 different organisms with one 20% smaller than the other. The data looks good for both and surprisingly the Bayesian molecular weight is not only similar but exactly the s...
by EduardoBruch
2018.12.02 19:46
Forum: Primary data processing
Topic: Molecular weight estimation
Replies: 5
Views: 6122

Re: Molecular weight estimation

That is exactly what I needed, about the version I am using the 2.8.3 in mac.
Thanks Al!
by EduardoBruch
2018.11.26 14:55
Forum: Primary data processing
Topic: Molecular weight estimation
Replies: 5
Views: 6122

Molecular weight estimation

Hello, For my usual data proccesing I was allways using the BSA standard or the Porod Volume/1.6 to estimate MW. I just started to used the new version of Primus (from 2.8.3 package) and I found the new Molecular weight analysis option very useful. I was wondering if there was any help/manual with f...