EOM on disordered peptides

Linear (OLIGOMER), and non-linear (MIXTURE) analysis, singular value decomposition (SVDPLOT), addition of missing fragments (BUNCH, CORAL), analysis of flexible systems (EOM/RANCH & GAJOE), flexible refinement of high-resolution models (SREFLEX)
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EOM on disordered peptides

#1 Post by nb_1993 » 2018.10.05 09:37

Dear all,

Recently I had performed TR-WAXS experiments on peptide solutions. These peptides have been synthesized from an FG-Nup protein which is an IDP. I am new to ATSAS software, and I tried using EOM for the analysis of the data.
Regarding the software, the data plots that i obtained at different time plots are dS(q,t) vs q plots. I also have the intensity plots (I vs q). I tried using both the data sets for analysis in EOM and i obtained different results. Which data sets must i use for the analysis?
Rg_distr_001_1 (1).png
Rg_distr_001_1 (1).png (3.7 KiB) Viewed 4683 times
Rg_distr_001_1.png (5.71 KiB) Viewed 4683 times

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use I(q) vs. q data files

#2 Post by AL » 2018.11.14 19:06

You should use I(q) vs. q background-subtracted experimental data files (*.dat) in ASCII format containing 3 columns: (1) momentum transfer q, (2) experimental intensity I(q) and (3) experimental errors.
Before interpreting the Rg histograms make sure the fit to your data is good.

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