Scaling intensities from CRYSON

Calculation of SAXS and SANS profiles (CRYSOL, CRYSON), superposition of models (SUPCOMB, DAMAVER, DAMCLUST), database DARA
Post Reply
Posts: 8
Joined: 2013.04.03 19:11

Scaling intensities from CRYSON

#1 Post by dggreene » 2013.04.11 01:16

I am trying to put the calculated intensity on the same scale as my data. From my understanding, the calculated intensity has units of cm^2 and it is the scattering cross-sectional area for one molecule, taking the solvent and hydration layer into account. To convert to absolute intensity (1/cm) I multiplied the intensity by the protein number density of my sample. However, there is a large discrepancy. The calculated intensity in on on order 10^7 cm^2 and the number density is on order 10^16 molecules/mL but my data are on order 10^-1. What other proportionality constant am I missing?


Active member
Posts: 22
Joined: 2009.01.28 00:06

Re: Scaling intensities from CRYSON

#2 Post by Andrew » 2016.08.17 01:26

Probably not useful for you, but perhaps someone else.

I have noticed this before, and I think that I came to the conclusion that there was a factor of 1E-24 (which fits with what you have here). The factor is likely due to a volume conversion from Angstroms^3 to cm^3 has not been applied correctly.

Post Reply