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Small Angle X-ray Scattering Initiative for Europe :: Forum
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PostPosted: 2016.10.12 16:22 
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Joined: 2016.09.20 16:54
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Hello all,

I've generated different 'envelopes' from SAXS data that are different deletion constructions of the same protein, ie I have data from the full length protein and 2 constructions that are missing different parts of the aa sequence. All 3 constructions have at least 1 part of the sequence (1 domain) in common.
So I was wondering, is there a way to align these 3 envelopes so I could identify the common domain (other than manually trying to fit them together)?

Thank you in advance.
Eleni.


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PostPosted: 2016.10.14 08:19 
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Location: EMBL Hamburg
I don't think there's any way you can do that with envelopes that you have already calculated. However, you can calculate new pre-aligned dummy atom models using MONSA. For the deletion constructs, just tell the program that the deleted domain is "contrast-matched" out.


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PostPosted: 2016.10.24 18:37 
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Thanks a lot!
This is actually better than a simple 'superposition'.


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