Hello,
I am wondering what is the overall experience with N-linked glycosylation contribution to the ab initio model - i.e. how much will be the glycan visible in the shape of the envelope. I am assuming that when the N-linked glycosylation is more or less flexible it wont be "visible" in the shape of the envelope, similarly to electron densities in high-resolution structures. But when the glycan is stabilized and rigid than it will be more significantly pronounced in the ab initio envelope.
Are these correct assumptions?