Small Angle X-ray Scattering Initiative for Europe :: Forum
It is currently 2018.05.23 22:28

All times are UTC + 2 hours

Post new topic Reply to topic  [ 2 posts ] 
Author Message
PostPosted: 2018.01.24 06:05 

Joined: 2018.01.24 05:34
Posts: 1
Hello, I am a graduate student who is trying to introduce SAXS to my laboratory. I have collected some data and processed it using the techniques I found on this forum. My project involves measuring a large conformational change in my protein of interest, which causes a slight change (0.5-2 angstroms) in Rg and a more notable change at 0.15 < q < 0.2 angstrom-1.

My mentor often asks about the reproducibility of the data, and inquires about replicate measurements of the same sample, such as would often be done in biology. I have the impression that this is not commonly done in SAXS experiments, but I do not know how to explain this to my mentor. It is hard to argue that more data is bad, even if it may be impractical.

I would appreciate any guidance on this topic. I have not found publications that discuss this topic but welcome any and all referrals. Thank you for your help.

PostPosted: 2018.01.31 23:55 
Active member

Joined: 2011.08.15 22:18
Posts: 20
Location: UTMB, Galveston, TX

In the simplest sense the standard dilution series is a replicate set, which demonstrates the reproducibility and concentration independence of your data. However, it does not account for sample preparation variation. Contaminants and buffer mismatch problems should vary between sample batches, which is a good reason to collect SAXS on more that one sample prep, but this is rarely done, unless the first prep shows sample pathologies in the SAXS data.

Best regards,

Display posts from previous:  Sort by  
Post new topic Reply to topic  [ 2 posts ] 

All times are UTC + 2 hours

Who is online

Users browsing this forum: No registered users and 2 guests

You cannot post new topics in this forum
You cannot reply to topics in this forum
You cannot edit your posts in this forum
You cannot delete your posts in this forum
You cannot post attachments in this forum

Search for:
Jump to:  
Powered by phpBB® Forum Software © phpBB Group