glycerol effect in measure

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PhD_fm
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Joined: 2012.05.08 11:58

glycerol effect in measure

#1 Post by PhD_fm » 2013.05.10 16:24

I have done several measure of same protein sample but with two buffer with only difference in the glycerol: without and with glycerol (10%). Although I don 't see any aggregation effect in the curves the MW estimation is totally opposite, one is half than I expected and the other is double.
I can not understand what it is happened. Are there any limit in glycerol concentration in the buffer??

Thank you in advances.

Alex
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Posts: 692
Joined: 2007.08.09 21:10
Location: Planet Earth

Re: glycerol effect in measure

#2 Post by Alex » 2013.05.16 18:12

hi,
one of my papers might be of interest to you:
http://www.ncbi.nlm.nih.gov/pubmed/22051511

Alex

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