DATVC for RNA data

Interactive and automated data processing and analysis tools: PRIMUS, CHROMIXS, GNOM, AUTORG, DATCMP, DATOP etc.
Post Reply
Message
Author
User avatar
Guðmundsdóttir
Member
Posts: 8
Joined: 2008.10.13 17:54
Location: Reykjavík, Iceland

DATVC for RNA data

#1 Post by Guðmundsdóttir » 2021.07.19 16:43

Hi all,

DATVC output is for protein data, right? Is there a way of estimating Vc MW for RNA data - as described in the original (Rambo & Tainer, 2013) paper?

Many thanks.

User avatar
AL
Administrator
Posts: 747
Joined: 2007.08.03 18:55
Location: EMBL Hamburg, Germany
Contact:

Re: DATVC for RNA data

#2 Post by AL » 2021.07.21 16:23

Guðmundsdóttir wrote:
2021.07.19 16:43
DATVC output is for protein data, right?
Yes. MW is calculated as

mass = (QR / ec)L

where QR = Vc2/Rg.
According to (Rambo & Tainer, 2013) Figure 3a, for proteins ec = 0.1231 and L = 1.0. These values are used in DATVC.
According to Figure 3b, for RNA ec = 0.00934 and L = 0.808. Thus you can calculate Vc MW for RNA data as

MWRNA = (0.1231*MWprotein/0.00934)0.808

Post Reply