Calculating Rg from in primus for disordered proteins

Interactive and automated data processing tools (PRIMUS, GNOM, AUTORG).
Scattering from simple bodies (BODIES), peak analysis (PEAK), data plotting (SASPLOT) etc.
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Oystein
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Calculating Rg from in primus for disordered proteins

#1 Post by Oystein » 2017.06.13 09:07

Hi

I am trying to calculate the Rg for a monodispersive intrinsically disordered protein. I loaded the subtracted average dat file by using Select in primus. Then i figured out i might use gnome to calculated Rg.

Gnome wants me to input the Rmax. But which value should i put here?

Help would be appreciated :)

Oystein

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AL
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use PRIMUS/Qt

#2 Post by AL » 2017.06.19 12:10

Oystein wrote:I loaded the subtracted average dat file by using Select in primus...
It looks like you are using the old version of PRIMUS (primusqw.exe) on Windows. Try the new cross-platform version (primusqt): go to 'Start > ATSAS 2.8.1 > SAS Data Analysis'. PRIMUS/Qt will open. Go to 'File > Open' to open your data. Then go to the 'Analysis' tab (bottom right corner of the window) and click 'Radius of Gyration' to estimate Rg using the Guinier approximation or click 'Distance Distribution' for the GNOM GUI.

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