SAS database for biological macromolecules

Small angle scattering software (except ATSAS)
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fjzhang
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SAS database for biological macromolecules

#1 Post by fjzhang » 2010.08.19 10:46

Due to some controversial reports on the equilibrium clustering in protein solutions, people suggest to build a SAS database like protein-data-bank for protein crystal structure information. This is absolutely a good idea.
Here my question is: what is the criterion for such a database?
I list here some factors need to be considered:
1) q range, depending on the size of the molecules and the interaction, the q range should be large enough for reliable data fitting;
2) statistics, or error bar given by the square root of intensity?
3) correct background subtraction
4) absolute intensity calibration
5) precise concentration of the macromolecules

Any further suggestion?
welcome comments on how to fulfill each criterion.

fjzhang
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Location: Tübingen University

other factors

#2 Post by fjzhang » 2010.08.19 11:07

such like the source of biomolecules, purity,
pH of the solutions, type of buffer,
ionic strength, type of salt

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AL
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Proposed requirements for a SAXS/SANS PDB entry

#3 Post by AL » 2010.11.11 18:48

You may find interesting the slides from the talk Validation of biomacromolecular structures by Gerard Kleywegt, see "Proposed requirements for a SAXS/SANS PDB entry".
Also have a look at the BIOISIS database.
Besides there is a move towards using ISPYB for SAXS experiments.

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AL
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SASBDB: data bank for biological small angle scattering data

#4 Post by AL » 2014.07.07 11:14


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