Contrasts for MONSA

Ab initio modelling: DAMMIF, DAMMIN, GASBOR, MONSA
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greatscott
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Joined: 2007.09.28 17:51
Location: NCMH, Nottingham Uni, UK.

Contrasts for MONSA

#1 Post by greatscott » 2007.10.01 17:31

Hello,
In the .con file, you need to input contrasts for each phase. I have a protein/DNA complex. What is the correct values for 0%, 65% and 100% for a nondeuterated "typical" protein/DNA complex?
It maybe useful to have a table of this data, for common phases (proteins, DNA, RNA, lipids) and measurements (both SAXS and SANS)
regards
Dave Scott.

SaxsMax
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#2 Post by SaxsMax » 2007.10.01 18:21

Hello,

For SAXS it is reasonable to use contrast =1 for the protein and 2 for DNA/RNA

In case of neutrons you can compute the contrast from arbitrary protein/nucleic acid by CRYSON and then use these numbers in Monsa
assuming they are the same for your case.
For lipids these values can be very different depending on the chemical composition.

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