CORAL-Domain fixation incompatible

Interactive modelling (MASSHA, SASpy) and global minimization programs (SASREF, BUNCH, CORAL, GLOBSYMM)
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msujata92
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Joined: 2019.01.08 18:00

CORAL-Domain fixation incompatible

#1 Post by msujata92 » 2019.01.08 18:15

I am trying to do rigid body modeling for an antibody and have 6 domains.
.con file is
L1.pdb
FabH.pdb
link 19
constantFC_A.pdb
L3.pdb
FabK.pdb
link 19
constantFC_B.pdb

The domains are extracted from the homology model (which has been done by H++ server) of the original sequence.
I am always getting the error that 'Domain 6 fixation is incompatible with the initial configuration' after grouping the domains. I am grouping (1,2), (4,5) and (3,6) domains.
I tried to move the FC domain little close to the Fabs, and also increase the number of linkers to 22 (which is wrong, because it has only 19) but getting chi2 as more than 85.xxx which is really bad.
Please guide me how to solve the issue.
Thank you in advance.

Also would like know how the coral online works?
how to give .cnd and .con file as input while submitting online?

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